Day 2 :
Lithuanian University of Health Sciences, Lithuania
Keynote: Scientific highlights of the cardiology department of the Lithuanian University of Health Sciences
Time : 9:00-9:45
Remigijus Žaliūnas is an Academician, Professor, Habilitated Doctor (biomedical sciences, medicine), Full Member of the Lithuanian Academy of Sciences, Rector of the Lithuanian University of Health Sciences, Head of the Department of Cardiology at the Medical Academy of the Lithuanian University of Health Sciences, Chairman of the Kaunas Region Society of Cardiology, President of the Lithuanian Tennis Union of Physicians, Member of the Executive Committee of the Lithuanian Student Basketball League, Member of Kaunas City Council of Academic Affairs, Member of the Board of the Association Santaka Valley, Doctor Honoris Causa of Moldova State University of Medicine and Pharmacy “Nicolae Testemitanu”, and an Honorary Consul of France in Kaunas. He has defended his Doctoral thesis in 1990 and his Habilitation thesis in 1995. In 1996, he was awarded the degree of an Associate Professor, and in 1999, the degree of a Professor. He is the author of 266 scientific publications, co-author of eight textbooks and seven teaching books.
Clinical and teaching activities of the Cardiology Department of the Lithuanian University of Health Sciences are inter-related with extensive scientific research. Scientific studies include joint research projects with other scientific – Research or Clinical Departments of the Lithuanian University of Health Sciences, such as Molecular Genetic laboratory, Epidemiologic laboratory, Ophthalmology and Radiology Departments, etc. Epidemiologic genetic studies include evaluation of the frequency of allele variants, investigations of 5A allele and its impact on the remodeling of ischemic myocardium. Genetic studies are focused on the analysis of the impact of SCARB1 single nucleotide polymorphism (SNP) rs5888 with plasma lipid profile and association with CAD in Lithuanian population characterized by high morbidity and mortality from CAD, on the search of molecular biomarkers to identify patients at risk for sporadic ascending aortic aneurysm and aortic dissection and on pharmacogenomics of anticoagulants and antiplatelets. A prospective cohort study is carried out for evaluation of links between cardio-vascular changes and genetic, metabolic and lifestyle cardiovascular risk factors in middle-aged individuals. MRI and echocardiographic studies include evaluation of left ventricular mechanics in functional ischemic mitral regurgitation in acute inferior myocardial infarction, comparison of dobutamine stress speckle tracking echocardiography and adenosine stress MRI in assessing significant coronary artery stenosis in patients with moderate and high probability of CAD, search of predictors of positive response to cardiac resynchronization therapy, evaluation of MRI feasibility to determine myocardial scar transmurality using the functional parameters alone. Two big studies are carried out in collaboration with foreign partners: “A clinical trial for the assessment of safety and efficacy of the laser wire ablation for the interventional treatment of paroxysmal atrial fibrillation. A first in human feasibility trial” and FP7-HEALTH-2013-INOVATION project “DISCHARGE – Diagnostic imaging strategies for patients with stable chest pain and intermediate risk of coronary artery disease: comparative effectiveness research of existing technologies”.
Baylor College of Medicine & University of Texas Medical Branch, USA
Time : 9:45-10:30
Yochai Birnbaum has completed his MD from the Hebrew University of Jerusalem, Israel. He is the John S Dunn Chair, Professor of Medicine at Baylor College of Medicine, Houston, Texas, USA. He has published more than 320 papers in reputed journals and has been serving as an Editorial Board Member of six journals.
Yumei Ye has completed her MD at the age of 24 years from Nanjing University of Traditional Chinese Medicine, Nanjing China. She is an Associate Professor at The Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, Texas. She has published more than 40 papers in reputed journals.
We assessed whether the SGLT-2 inhibitor dapagliflozin (Dapa) attenuates the upregulation of the cardiac Na+/H+ exchanger (NHE-1) in-vitro in mouse cardiofibroblasts stimulated with lipopolysaccharides (LPS) and whether this effect is dependent on adenosine monophosphate kinase (AMPK) activation. Mouse cardiofibroblasts were exposed for 16 hours to Dapa (0.4 μM), AMPK activator [A769662 (10 μM)], AMPK inhibitor [compound C (CC) (10 μM), an SGLT1 and SGLT2 inhibitor [phlorizin (PZ) (100 μM)], Dapa+CC, or Dapa+PZ, and then stimulated with LPS (10 ng/ml) for three hours. NHE-1 mRNA levels were assessed by rt-PCR and total AMPK, phosphorylated-AMPK (P-AMPK), NHE-1 and heat shock protein-70 (Hsp70) protein levels in the whole cell lysate by immunoblotting. In addition, NHE-1 protein levels attached to Hsp70 were assessed by immunoprecipitation. Exposure to LPS significantly reduced P-AMPK levels in the cardiofibroblasts. A769662 and Dapa equally increased P-AMPK. The effect was blocked by CC. Phlorizin had no effect on P-AMPK. LPS exposure significantly increased NHE-1 mRNA levels. Both Dapa and A769662 equally attenuated this increase. The effect of Dapa was blocked with CC. Interestingly, none of the compounds significantly affected NHE-1 and Hsp70 protein levels in the whole cell lysate. However, LPS significantly increased the concentration of NHE-1 attached to Hsp70. Both Dapa and A769662 attenuated this association and CC blocked the effect of Dapa. Again, phlorizin had no effect and did not alter the effect of Dapa. Dapa increases P-AMPK in cardiofibroblasts exposed to LPS. Dapa attenuated the increase in NHE-1 mRNA and the association between NHE-1 and Hsp70. This effect was dependent on AMPK.